Ssr and Srap Marker Based Linkage Map of Vitis Amurensis Rupr

نویسندگان

  • ZHENDONG LIU
  • XIUWU GUO
  • YINSHAN GUO
  • HONG LIN
  • PENGXIANG ZHANG
  • YUHUI ZHAO
  • KUN LI
  • CHENGXIANG LI
چکیده

SSR (Simple Sequence Repeat) markers and SRAP (Sequence Related Amplified Polymorphism) markers were used to construct a genetic linkage map of Vitis amurensis Rupr. The mapping population consists of 94 individuals derived from the selfing of Beibinghong, a V. amurensis cultivar. The map covers a total length of 1123cM on 21 linkage groups with an average distance between adjacent markers of 4.5cM and 10.9 markers per linkage group on average. The order of SSR markers in the map presented in this paper was mainly consistent with the previous published Vitis linkage maps except for few inversions. The efficiency of SSRs to anchor homologous linkage groups from different Vitis maps and the ability of SRAPs in saturating molecular linkage maps were observed. This map can serve as a fundamental tool for a series of further studies in V. amurensis, including resistance trait QTL (quantitative trait locus) detection and MAS (marker assisted selection).

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Using specific length amplified fragment sequencing to construct the high-density genetic map for Vitis (Vitis vinifera L. × Vitis amurensis Rupr.)

In this study, 149 F1 plants from the interspecific cross between 'Red Globe' (Vitis vinifera L.) and 'Shuangyou' (Vitis amurensis Rupr.) and the parent were used to construct a molecular genetic linkage map by using the specific length amplified fragment sequencing technique. DNA sequencing generated 41.282 Gb data consisting of 206,411,693 paired-end reads. The average sequencing depths were ...

متن کامل

SSR and SRAP marker-based linkage map of Vitis vinifera L.

An F1 population was created by the cross '87-1' × '9-22'. The female parent '87-1' was an extremely early maturing cultivar with strong flavour. The male parent was an excellent breeding line producing large berries maturing late. The mapping population included 149 randomly chosen individuals. Molecular genetic map for each parent and the consensus map were constructed using simple sequence r...

متن کامل

Genetic linkage map of EST-SSR and SRAP markers in the endangered Chinese endemic herb Dendrobium (Orchidaceae).

Dendrobium officinale is an endangered orchid from southeast Asia that is known for its medicinal properties in traditional Chinese medicine. We constructed an integrated genetic linkage map of an F(1) population derived from an interspecific cross between D. officinale and D. aduncum (both, 2n = 38), using expressed sequence tag-simple sequence repeats (EST-SSR) and sequence-related amplified ...

متن کامل

[Molecular mapping of the gene(s) controlling petal-loss trait in Brassica napus].

An F2 population derived from a cross between apetalous line' APT02 'and normal petalled cultivar 'ZS NO.4' was used for molecular marker searching and chromosomal mapping of the gene(s) controlling petal-loss trait in Brassica napus. Twenty pairs of AFLP primers and 170 pairs of SRAP primers were selected and screened from two parents. In further selection through bulked segregant analysis (BS...

متن کامل

Genetic Diversity of Chinese Wild Grape Species by Ssr and Srap Markers

Simple Sequence Repeats (SSR) and Sequence-Related Amplified Polymorphism (SRAP) were used to analyze the genetic diversity of 26 genotypes from 15 Chinese wild grape species and one interspecific hybrid of Vitis L. A total of 90 alleles were amplified by ten SSR primers, and primer heterozygosity varied from 0.653 to 0.883. Twelve out of 169 SRAP primer combinations were selected for SRAP anal...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2013